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POSTERS: Late Breaking I

P-653 - Comparative evaluation of a new Bacillus-based biofungicide with commercial standards for efficacy, metabolite content, and formulation quality

Monday, August 4, 2025
4:30 PM - 5:15 PM HST
Location: Exhibit Hall 3

    Presenting author(s)

  • JC

    Julia M. Crane

    Manager Crop Protection Research
    Valent BioSciences
    Libertyville, Illinois, United States

    • Author(s)

  • BW

    Bill Weldon

    Plant Pathologist
    Valent BioSciences
    Libertyville, Illinois, United States

  • DZ

    Daniel Zommick

    Valent BioSciences
    Libertyville, Illinois, United States

  • GV

    Gabrielle Valenzuela

    Valent BioSciences
    Libertyville, Illinois, United States

  • LE

    Luis Espinosa

    Valent BioSciences
    Libertyville, Illinois, United States

  • IS

    Ivy Sun

    Valent BioSciences
    Libertyville, Illinois, United States

  • AP

    Anissa Poleatewich

    Mycorrhizal Applications
    Grants Pass, Oregon, United States

Abstract Text:

Amyloshield® WG Biological Fungicide, with the active ingredient Bacillus amyloliquefaciens strain PTA-4838, offers multiple modes of action for fungal and bacterial disease management. This study compared AmyloShield WG with other commercial Bacillus-based biofungicides with respect to bioefficacy, metabolite content, and formulation characteristics. An in vitro growth inhibition bioassay assessed comparative efficacy against Botrytis cinerea (gray mold), in which AmyloShield WG demonstrated greater growth suppression at concentrations reflecting lowest label rates. Chemical analysis compared cyclic lipopeptide (CLP) profiles and concentrations, specifically surfactin, fengycin and iturin A, spore and total cell counts before and after storage, and formulation characteristics such as solubility. AmyloShield WG provided the highest concentration of total CLPs when extrapolated to label field rates and a larger proportion of fengycin relative to the other biofungicides. AmyloShield WG also exhibited spore stability at storage temperatures up to 40oC for 18 weeks. AmyloShield WG represents a significant advancement in biological disease management with more consistent efficacy compared to the other biofungicides tested, which may be related to differences in the quantity and ratio of CLPs, or the formulation quality.