P-278 - Association mapping to identify adult plant resistance loci to stripe rust in hard winter wheat

Tuesday, August 5, 2025

9:30 AM - 10:15 AM HST

Location: Exhibit Hall 3

Presenting Author(s)

MA

Meriem Aoun

Oklahoma State University
Stillwater, Oklahoma, United States

Author(s)

MM

Mushfique Arefin Mobin

Oklahoma State University
Stillwater, Oklahoma, United States
RS

Rajat Sharma

Graduate Research Assistant
Oklahoma State University
Stillwater, OK, USA
BC

Brett F. Carver

Rgts Prof/Enwd Ch
Oklahoma State University
Stillwater, Oklahoma, United States
KG

Kimberly Garland Campbell

USDA-ARS Wheat Health, Genetics, and Quality Research Unit
Pullman, Washington, United States
MG

Mary Guttieri

USDA-ARS Hard Winter Wheat Genetics Research Unit
Manhattan, Kansas, United States
PS

Paul St. Amand

USDA-ARS Hard Winter Wheat Genetics Research Unit
Manhattan, Kansas, United States
AB

Amy Bernardo

USDA-ARS Hard Winter Wheat Genetics Research Unit
Manhattan, Kansas, United States
GB

Guihua Bai

USDA-ARS Hard Winter Wheat Genetics Research Unit
Manhattan, Kansas, United States
IL

Indira Priyadarshini Lakkakula

Graduate Student
Kansas State University
Manhattan, Kansas, United States
AA

Anju Maan Ara

Graduate Research Assistant
Oklahoma State University
Stillwater, Oklahoma, United States

Abstract Text: Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a major threat to wheat production. To identify resistance genes in contemporary hard winter wheat, we evaluated 619 breeding lines and cultivars from the Oklahoma State University wheat breeding program at the seedling stage against race PSTv-37 and at the adult plant stage in Oklahoma, Kansas, and Washington. The panel was genotyped using genotyping-by-sequencing (GBS) and markers linked to nine known stripe rust resistance (Yr) genes: Yr5, Yr15, Yr17, Yr18, Yr29, Yr36, Yr40, Yr46, and QYr.tamu-2B. Genome-wide association studies (GWAS) were conducted using phenotypic data, single nucleotide polymorphism (SNP) markers generated from GBS, and markers linked to Yr genes. Only 2% of genotypes were resistant at the seedling stage, whereas 51–76% showed resistance at the adult plant stage across field environments. Based on gene-linked markers, Yr17, Yr18, Yr29, and QYr.tamu-2B were present in 48%, 17%, 61%, and 13% of the genotypes, respectively, while the other five genes were absent. GWAS identified significant adult plant resistance loci on chromosome arms 2DL, 3BS, and 4BL across multiple field environments. Significant SNPs in these three genomic regions were converted into kompetitive allele-specific PCR (KASP) markers to facilitate their deployment in breeding. These findings offer opportunities to enhance and diversify stripe rust resistance in hard winter wheat.