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POSTERS: Late Breaking II

P-660 - Identification and Pathogenicity of Fungal Trunk Pathogens Obtained from Airborne Spores in Commercial Vineyards in Central Chile

Tuesday, August 5, 2025
10:15 AM - 11:00 AM HST
Location: Exhibit Hall 3
Abstract Text:

Grapevine trunk diseases (GTDs) represent a significant threat to Chilean viticulture, with Botryosphaeriaceae fungi being the major causal agents. Airborne inoculum composition and monitoring are crucial for management strategies of GTDs. Therefore, this study was performed to identify and determine the pathogenicity of fungal trunk pathogens obtained as airborne inoculum. Weekly spore monitoring was conducted throughout 2024 using spore traps with vaseline (24 microscopic glass slides) in the Maule and Maipo valley vineyards. Captured spores were cultured on PDA and identified through morphological and molecular characteristics. The results identified Diplodia seriata (11 isolates), Dothiorella sarmentorum (two isolates) and Truncatella angustata (five isolates). The size of necrotic lesions of lignified cutting varied by species and isolates of Diplodia, Dothiorella, and Truncatella. Moreover, the isolates of D. seriata showed more variation in aggressiveness within populations. This research provides new insights into airborne fungal diversity affecting Chilean vineyards and establishes the foundation for targeted disease prevention strategies.