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POSTERS: Biological Control II

P-046 - Study on the application of Bacillus velezensis HL_B03 to control plant diseases and the analysis of its antifungal agents

Tuesday, August 5, 2025
9:30 AM - 10:15 AM HST
Location: Exhibit Hall 3

    Presenting Author(s)

  • CW

    Chia-Yi Wu, MA

    Assistant researcher
    Hualien District Agricultural Research and Extension Station MOA., R.O.C.(Taiwan)
    Hualien, Hualien, Taiwan (Republic of China)

    • Author(s)

  • JC

    Jhih-Jia Ciou

    Research assistant
    Hualien District Agricultural Research and Extension Station MOA., R.O.C.(Taiwan)
    Hualien, Hualien, Taiwan (Republic of China)

  • WT

    Wei-An Tsai

    Former assistant researcher
    Hualien District Agricultural Research and Extension Station MOA., R.O.C.(Taiwan)
    Hualien, Hualien, Taiwan (Republic of China)

  • YT

    Yi-Chen Tsai

    Associate researcher
    Hualien District Agricultural Research and Extension Station MOA., R.O.C.(Taiwan)
    Hualien, Hualien, Taiwan (Republic of China)

  • SC

    Shu-Yuan Chiou

    Researcher
    Hualien District Agricultural Research and Extension Station MOA., R.O.C.(Taiwan)
    Hualien, Hualien, Taiwan (Republic of China)

  • TY

    Ta-Chi Yang

    Director
    Hualien District Agricultural Research and Extension Station MOA., R.O.C.(Taiwan)
    Hualien, Hualien, Taiwan (Republic of China)

  • YC

    Yi-Jyun Chen

    Agricultural Biotechnology Research Center, Academia Sinica, Taiwan
    Tainan, Tainan, Taiwan (Republic of China)

  • CL

    Ching-Chih Lin

    Agricultural Biotechnology Research Center, Academia Sinica, Taiwan
    Tainan, Tainan, Taiwan (Republic of China)

  • WH

    Wei-Chen Hsu

    Agricultural Biotechnology Research Center, Academia Sinica, Taiwan
    Tainan, Tainan, Taiwan (Republic of China)

  • BW

    Bo-Wei Wang

    Agricultural Biotechnology Research Center, Academia Sinica, Taiwan
    Tainan, Tainan, Taiwan (Republic of China)

  • YY

    Yu-Liang Yang

    Associate research fellow
    Agricultural Biotechnology Research Center, Academia Sinica, Taiwan
    Tainan, Tainan, Taiwan (Republic of China)

Abstract Text:

Bacillus velezensis HL_B03 was a beneficial bacteria isolated from the soil in cabbage field in Hualien. We cultured the serious fungal pathogens that happened commonly in Hualien in confrontation with HL_B03. Results showed that HL_B03 had good inhibitory effects, over 50%, on growth of gummy blight (Stagonosporopsis cucurbitacearum), welsh onion black rot (Stemphylium vesicarium) and blueberry anthracnose (Colletotrichum fructicola). In pot experiments, welsh onions were treated with HL_B03 broth and then inoculated with S. vesicarium. On the variety ‘Lazy’, the lesions in HL_B03 group were 2.3mm, which was significantly smaller than the one in water treatment group. HL_B03 also has the characteristic of high temperature resistance, and it can be mixed with rapeseed meal to be used as microbial fertilizer. In the controlling assay of Fusarium oxysporum f.sp. luffae on loofah, the treatment of HL_B03-rapeseed meal mix could reduce disease incidence by 50% compared to untreated group. To confirm the antifungal substances in metabolites, the broth was purified by MPLC. Iturin A2, A3, and A6 were major ones of iturins, C-16 fengycin A and C-16 fengycin B were dominant in fengycins. The antifungal assay indicated that iturins were active against F. oxysporum f.sp. luffae LA1 (MIC: 50μg/disc). Then we dropped the broth of HL_B03 next to LA1 spores suspension. By imaging mass spectrometry analysis, iturins were detected and distributed around the broth. No fengycins were observed. Based on the results, we can propose that iturins are the dominant antifungal agents of HL_B03.