Gonzalo A. Diaz, professor (he/him/his)
Ing. Agr. MS. Dr
Universidad de Talca
Talca, Maule, Chile
Yadira A. Hernandez, Yadira
PhD student
Universidad de Talca
Talca, Maule, Chile
Karina Elfar, Karina Elfar PhD (she/her/hers)
Project Scientist
University of California, Davis
Davis, California, United States
Akif Eskalen, Akif Eskalen
University of California, Davis
Davis, California, United States
Mauricio A. Lolas, Mauricio Lolas (he/him/his)
Ingeniero Agronomo, M.S., Ph.D.
Facultad de Ciencias Agrarias, Universidad de Talca
Talca, CHILE
Paulina A. Gonzalez, Paulina Gonzalez
Researcher
Universidad de Talca
Talca, Maule, Chile
Fernanda B. Nuñez, Fernanda
Researcher
Universidad de Talca
Talca, Maule, Chile
Botryosphaeria dieback is one of the most important grapevine trunk diseases (GTDs) in Central Chile. Pruning wounds serve as the primary entry point for these pathogens in vineyards. Despite increasing knowledge of the interaction between Botryosphaeriaceae species and grapevines, the duration for which pruning wounds remain susceptible under local climatic conditions in Chile is still unknown. This study aims to determine the susceptibility period of pruning wounds to Neofusicoccum isolates obtained from apples and grapevines in vineyards of the Maule Region. Experiments were conducted on rooted cuttings (greenhouse) and lignified canes (vineyard), using a mycelial fragment suspension of N. parvum (grapevine) and N. arbuti (apple). Inoculation was performed one day after pruning and at two-week intervals for 60 days. The results indicate that pruning wounds are more susceptible to infection by N. parvum and N. arbuti during the first 15 days, with lesion lengths of 119.6 and 83.1 mm in cuttings and 52.3 and 39.2 mm in vineyards. N. parvum causes significantly larger lesions than N. arbuti at all pruning wound ages. The susceptibility of pruning wounds persists up to 45 days, especially for N. parvum, while minor damage is still observed at 60 days, indicating prolonged vulnerability. These findings highlight the importance of protecting pruning wounds for an extended period to mitigate the risk of infection.