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POSTERS: Disease Detection and Diagnosis I

P-157 - Susceptibility of oat varieties to Wheat streak mosaic virus and Triticum mosaic virus

Monday, August 4, 2025
3:45 PM - 4:30 PM HST
Location: Exhibit Hall 3
Abstract Text: Wheat streak mosaic virus (WSMV) and Triticum mosaic virus (TriMV) are major production constraints of US wheat. Although WSMV has long been present in the US Great plains, TriMV was first reported in 2006 in Kansas wheat crop. Since then, both viruses have often been found in coinfection. Infected volunteer wheat is currently considered as the primary virus source for WSMV and TriMV dissemination by wheat curl mite. However, our recent study reported TriMV in field oats for the first time in 2023. A state-wide sampling in Nebraska further confirmed higher infection rates in oats compared to wheat for both WSMV and TriMV. Interestingly, oats are often used as a cover crop in the Great Plains wheat growing region. This suggests that oat crops can potentially act as a primary source of inoculum for both WSMV and TriMV. Currently, we have limited understanding on the susceptibility of oat varieties to both WSMV and TriMV. This study evaluated susceptibility of 10 commonly grown oat varieties in Nebraska to WSMV and TriMV. Five oat seedlings of each variety were mechanically inoculated with freshly prepared crude sap as a virus inoculum. Each treatment was repeated three times. Composite leaf samples were collected 17-days post-inoculation and assayed with both RT-PCR and ELISA. TriMV infection ranged from 20-100%, confirming susceptibility of all oat varieties to TriMV. Ongoing studies are assessing the susceptibility of oat varieties to WSMV, as well as vertical transmission of both WSMV and TriMV in first- and second-generation oat seed.