Skip to main content
APS Plant Health 2025 Main banner
Icon Legend
This session is not in your schedule.
This session is in your schedule. Click again to remove it.

POSTERS: Molecular/Cellular Plant‐Microbe Interactions I

P-363 - The presence of novel antiviral mechanisms against mycovirus and its induction for mycoviral clearance

Monday, August 4, 2025
4:30 PM - 5:15 PM HST
Location: Exhibit Hall 3

    Presenting Author(s)

  • JC

    Jeesun Chun

    Jeonbuk National University
    Jeonju, SOUTH KOREA

    • Author(s)

  • YK

    Yo-Han Ko

    Dr.
    Jeonbuk National University
    Jeonju-si, Cholla-bukto, Republic of Korea

  • KS

    Kum-Kang So

    Jeonbuk National University
    Jeonju, Cholla-bukto, Republic of Korea

  • DK

    Dae-Hyuk Kim

    Jeonbuk National University
    Jeonju, Cholla-bukto, Republic of Korea

Abstract Text: Mutation of DNA methyltransferases, DNMTI and DNMTII genes, essential for the epigenetic control of global gene expression in Cryphonectria parasitica, resulted in Cryphonectria hypovirus 1 (CHV1) clearance. To understand the molecular mechanism of viral clearance, we examined the general control nonderepressible 2 (GCN2) pathway, which regulates the phosphorylation of translation initiation factor 2α (eIF2α) as a well-known antiviral response in mammalian cells. CHV1 infection was found to suppress the GCN2 activity as indicated by phosphorylation level of eIF2α. However, mutations in DNMTII hyperactivated GCN2 activity, leading to viral clearance. When we mutated the GCN2 gene with the genetic background of DNMTII mutation, viral clearance was no longer observed, confirming its essential role as an antiviral response. Our findings reveal that GCN2 pathway functions as a novel antiviral response in C. parasitica. Moreover, our study demonstrate that CHV1 targets the GCN2 pathway to establish persistent infection, and that the synergistic activation of RNAi and GCN2 pathways is crucial for effective viral clearance in C. parasitica.