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POSTERS: Disease Detection and Diagnosis II

P-200 - Toward a sampling approach for accurate detection of Emaravirus rosae

Tuesday, August 5, 2025
9:30 AM - 10:15 AM HST
Location: Exhibit Hall 3

    Presenting Author(s)

  • FO

    Francisco M. Ochoa Corona

    Professor
    Oklahoma State University
    Stillwater, OK, USA

    • Author(s)

  • MM

    Meredith Mitchell

    Undergraduate student
    Oklahoma State University
    Stillwater, Oklahoma, United States

  • AR

    Arianna Ragona

    Doctoral Student
    University of Palermo, Palermo, ITALY
    Palermo, Sicilia, Italy

Abstract Text: Rose rosette disease (RRD) caused by Emaravirus roseae, formerly Rose rosette virus (RRV), threatens the rose industry in the U.S. and worldwide. RRV was reported in Canada (1940) and has spread throughout the continental U.S. Infected propagation tissue, lack of preventive eradication of infected plants, and the wind spreading of one of its eriophyid mite vectors, Phyllocoptes fructiphilus, are the main ways RRD spreads. Symptoms of RRD include abnormal red coloration of shoots and foliage, sprout elongation, excessive thorn proliferation, witches’ broom, elongated or misshapen leaves, mottled or distorted flowers, thick secondary branches compared to primary branches, and reduced resistance to winter; plants may die two or three years post-infection. However, roses infected with RRV may show little or no symptoms in the early stages of the infection, and the distribution of symptoms is not homogeneous. This study aims to gain insight into the distribution of RRV in infected plants regarding sampling purposes for application in areas where RRD is not reported. Rose plants were visually divided into four quadrants, and symptomatic and non-symptomatic leaves and young branches were sampled in each quadrant. The results show that using direct virus trapping in plastic and RRV RT-qPCR, sampling one non-symptomatic and one symptomatic branch in each of the empirically divided four sections of the plant to total eight samples per plant allows RRD detection. The first programmed samplings were made in late fall when the virus is expected to be low titer.