Skip to main content
APS Plant Health 2025 Main banner
Icon Legend
This session is not in your schedule.
This session is in your schedule. Click again to remove it.

POSTERS: Proteomics/Metabolomics/Genomics II

P-584 - Transcriptome analysis to uncover the host-response components associated with beet necrotic yellow vein virus infection in sugarbeet

Tuesday, August 5, 2025
10:15 AM - 11:00 AM HST
Location: Exhibit Hall 3

    Presenting Author(s)

  • CC

    Chinnaraja Chinnadurai

    Research Associate
    North Dakota State University
    Fargo, North Dakota, United States

    • Author(s)

  • AF

    Alyssa Flobinus

    North Dakota State University
    Fargo, North Dakota, United States

  • HC

    Hyun Cho

    USDA-ARS
    Fargo, North Dakota, United States

  • EB

    Eric Branch, PhD

    Assistant Professor
    North Dakota State University and University of Minnesota Extension
    Fargo, North Dakota, United States

  • JW

    John Weiland

    USDA-ARS
    Fargo, North Dakota, United States

  • MB

    Melvin Bolton, PhD

    Research Plant Pathologist
    USDA-Agricultural Research Service, Sugar beet Research Unit, Fargo, ND 58102
    Fargo, North Dakota, United States

  • VR

    Vanitharani Ramachandran

    Research Plant Pathologist
    USDA-ARS
    Fargo, North Dakota, United States

Abstract Text:

Beet necrotic yellow vein virus (BNYVV) is the causal agent of rhizomania disease of sugarbeet (Beta vulgaris L.), significantly impacting crop yield and quality. Understanding the molecular defense response of sugarbeet against BNYVV infection is important for developing resistant cultivars. This study employs transcriptome sequencing to identify differentially expressed genes (DEGs) involved in host defense responses. To conduct the experiment, in vitro RNA transcripts for BNYVV RNA1 to RNA4 were synthesized from linearized plasmid DNA clones and inoculated Chenopodium quinoa plants. Sap from the symptomatic C. quinoa leaves was then used to infect sugarbeet seedlings via root-inoculation. Root samples from both inoculated and control plants were collected at 2-, 4-, and 6-weeks post-inoculation, with four biological replicates for each time point. BNYVV infection in the inoculated samples was confirmed using ELISA. For transcriptome analysis, total RNA from root samples was analyzed using directional mRNA sequenced with Illumina NovaSeq PE150 platform. Ongoing bioinformatic analysis focuses on profiling differentially expressed host genes in response to BNYVV infection. The findings will enhance understanding the molecular basis of host response to BNYVV and suggest potential genetic targets for improving rhizomania disease management.