David Gramaje
Instituto de Ciencias de la Vid y del Vino
Logrono, La Rioja, Spain
Catarina Leal
Instituto de Ciencias de la Vid y del Vino
Logrono, La Rioja, Spain
Ales Eichmeier
Mendel University
Lednice, Moravskoslezsky kraj, Czech Republic
Maria Julia Carbone
Universidad de la Republica
Montevideo, Montevideo, Uruguay
Tomas Kiss
Mendel University
Lednice, Moravskoslezsky kraj, Czech Republic
Dorota Tekielska
Mendel University
Lednice, Moravskoslezsky kraj, Czech Republic
Rebeca Bujanda
Instituto de Ciencias de la Vid y del Vino
Logrono, La Rioja, Spain
Dactylonectria macrodidyma, a key pathogen causing black foot disease in grapevines, alters soil microbiomes, yet its impact on fungal community dynamics across different soil types remains unclear. Understanding these interactions is crucial for developing soil-specific disease management strategies. This study evaluates how D. macrodidyma modifies fungal diversity, composition, and network interactions in grapevine roots and rhizosphere across sandy and clay soils. Tempranillo grapevines grafted onto 110 Richter rootstock were grown in controlled greenhouse conditions with sandy or clay soils and inoculated with D. macrodidyma. High-throughput sequencing, microbial network analysis, and functional guild predictions were performed at multiple time points. D. macrodidyma reduced fungal diversity in root microbiomes, particularly in sandy soil. It displaced beneficial fungi (Clonostachys, Trichoderma) while promoting pathogens (Ilyonectria, Botrytis). Network analysis revealed increased competition in sandy soil but cooperative pathogenic interactions in clay soil, leading to a shift toward pathogenic dominance. This study highlights the soil-dependent impact of D. macrodidyma on grapevine microbiomes and underscores the need for tailored disease management strategies. Soil amendments and biocontrol applications could mitigate pathogen-driven disruptions, enhancing microbial resilience and plant health.