Rajat Sharma
Graduate Research Assistant
Oklahoma State University
Stillwater, OK, USA
Meinan Wang
Research Associate
Washington State University
Pullman, Washington, United States
Xianming Chen
USDA-ARS Wheat Health, Genetics, and Quality Research Unit
Pullman, Washington, United States
Brett F. Carver
Rgts Prof/Enwd Ch
Oklahoma State University
Stillwater, Oklahoma, United States
Mary Guttieri
USDA-ARS Hard Winter Wheat Genetics Research Unit
Manhattan, Kansas, United States
Paul St. Amand
USDA-ARS Hard Winter Wheat Genetics Research Unit
Manhattan, Kansas, United States
Amy Bernardo
USDA-ARS Hard Winter Wheat Genetics Research Unit
Manhattan, Kansas, United States
Guihua Bai
USDA-ARS Hard Winter Wheat Genetics Research Unit
Manhattan, Kansas, United States
Anju Maan Ara
Graduate Research Assistant
Oklahoma State University
Stillwater, Oklahoma, United States
Meriem Aoun
Oklahoma State University
Stillwater, Oklahoma, United States
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is considered the most damaging disease in the heart of the U.S. hard winter wheat (HWW) region of Oklahoma and Kansas. Identifying resistance genes is crucial for further development of resistant cultivars. Baker’s Ann is a HWW cultivar released in 2018 by the Oklahoma State University wheat breeding program. Although susceptible at the seedling stage, Baker’s Ann has exhibited stable and high levels of stripe rust resistance at the adult plant stage since 2014. The objective of this study was to identify quantitative trait loci (QTL) underlying stripe rust adult plant resistance in Baker’s Ann. A total of 125 doubled haploid (DH) lines, derived from the cross OK12D22004-016 × Baker’s Ann, were field-evaluated at the adult plant stage in Oklahoma, Kansas, and Washington. The DH population was genotyped using genotyping-by-sequencing, and 10,135 polymorphic single-nucleotide polymorphisms (SNP) markers were used for genetic mapping. Four QTLs were detected, two of which had major effects, with one on chromosome 2DL from Baker’s Ann, and another one linked to Yr17 on 2AS from OK12D22004-016, explaining 33–58% and 17–20% of the phenotypic variation, respectively. The QTL on 2DL was detected in all environments, whereas the QTL on 2AS was identified at only Mt. Vernon, WA. Minor QTLs were mapped on chromosome 4BL from Baker’s Ann and 2AL from OK12D22004-016. The SNPs flanking the major QTL from Baker’s Ann were converted into kompetitive allele-specific PCR markers to facilitate marker-assisted selection.