Jiawen Wen
Graduate Research Assistant
University of Florida Citrus Research and Education Center
Lake Alfred, Florida, United States
Connor Hendrich
University of Florida Citrus Research and Education Center
Lake Alfred, Florida, United States
Jin Xu
University of Florida Citrus Research and Education Center
Lake Alfred, Florida, United States
Zhiqian Pang
University of Florida Citrus Research and Education Center
Lake Alfred, Florida, United States
Nian Wang
Professor
University of Florida
Lake Alfred, FL, USA
Citrus Huanglongbing (HLB) is a devastating disease worldwide. Most commercial citrus varieties are susceptible. However, Swinglea glutinosa, a citrus relative that can be used as a citrus rootstock, shows resistance to Candidatus Liberibacter asiaticus (CLas), the causative agent of HLB. Australian limes also show resistance to CLas. Here, we sequenced the haplotype-resolve genome of S. glutinosa. We further conducted comparative genomic analysis of 25 HLB-resistant and -susceptible citrus varieties and their relatives to reveal genetic mechanisms underlying resistance to HLB. A total of 40,756 gene families were identified, and 23.7% of the gene families were core gene families. We have analyzed unique genes in HLB resistant varieties with the focus on immune related genes including NLRs, antimicrobials, PR genes, and ROS genes. Our study provides valuable insights regarding HLB resistance mechanism, which will assist future citrus genetic improvement against HLB.