P-374 - Effect of Ca2+-regulated kinase CIPK10 on resistance response to soybean mosaic virus

Tuesday, August 5, 2025

10:15 AM - 11:00 AM HST

Location: Exhibit Hall 3

Author(s)

HC

Huong Cam Chu

Seoul National University
Seoul, Seoul-t'ukpyolsi, Republic of Korea
MA

Mazen Alazem

The Donald Danforth Plant Science Center
St. Louis, Missouri, United States
JY

Jisuk Yu

Seoul National University
Seoul, Seoul-t'ukpyolsi, Republic of Korea

Presenting Author(s)

KK

Kook-Hyung Kim

Seoul National University
Seoul, Seoul-t'ukpyolsi, Republic of Korea

Abstract Text: Calcineurin B-like (CPL)-interacting protein kinases (CIPKs) are a group of plant-specific kinases that interact with CBLs to regulate a wide range of downstream responses that affect plant growth and stress responses. In this study, we investigated the role of the CIPK10 in soybean resistance to soybean mosaic virus (SMV). Significantly increased resistance to SMV-G5H was observed in William82 plants overexpressing GmCIPK10. We constructed a mutant clone of GmCIPK10 that lacks the NAF domain, known as the CBL-interacting domain of CIPK, to investigate whether CBL-CIPK interaction is responsible for the SMV resistance response. Plants overexpressing the mutant showed higher SMV susceptibility than plants overexpressing wild-type CIPK10. Since overexpression of GmCIPK10 reduced SMV-G5H accumulation in the susceptible cultivar, a yeast two-hybrid assay was performed to examine the protein-protein interaction between GmCIPK10 and viral proteins. Surprisingly, GmCIPK10 did not interact with any of the SMV-G5H proteins. We then tried to find the partner of GmCIPK10 using Y2H, and GmCBL9 was chosen based on previous studies. Contrary to our expectation, GmCIPK10 and GmCBL9 did not interact with each other, and GmCBL9 did not interact with the viral proteins. Localization experiments showed that GmCIPK10 is located in the cytoplasm. Our results suggest that GmCIPK10 is involved in soybean immunity to SMV G5H, but the exact mechanism by which GmCIPK10 is involved is unknown. This work provides the first evidence for the effect of CIPKs on the antiviral resistance in soybean plants.