Sabrina D. Blanco (she/her/hers)
National Pingtung University of Science and Technology
Neipu, Pingtung, Taiwan (Republic of China)
Yi-Hsien Lin
Professor
National Pingtung Univ of Science and Tech
Pingtung, Pingtung, Taiwan (Republic of China)
Bacterial soft rot, caused by Pectobacterium carotovorum subsp. carotovorum (Pcc), is a serious disease in cabbage production. Bacillus amyloliquefaciens PMB05 fermentation liquid is effective in intensifying plant defense responses and controlling various plant bacterial diseases. Silicon can also improve disease resistance in plants. However, it is unknown whether the application of silicon with the B. amyloliquefaciens PMB05 fermentation liquid can increase its effectiveness in disease control. This study aimed to evaluate the regulatory effects of silicate treatments in B. amyloliquefaciens PMB05 on the intensification of cabbage immune responses upon pathogen-associated molecular patterns (PAMPs) recognition and their efficacy on disease resistance to bacterial soft rot in cabbage. First we demonstrated that the application of sodium silicate treatment did not increase the growth inhibition of PMB05 against Pcc. Additionally, the plant immune signal, such as reactive oxygen species (ROS), induced by HrpN were further intensified by the application of PMB05 in cabbage. Interestingly, we found that the application of different concentrations of sodium silicate with PMB05 bacterial suspension and fermentation liquid can enhance ROS generation and the control efficacy of bacterial soft rot disease. To conclude, silicate application can improve the mechanism behind B. amyloliquefaciens PMB05 fermentation liquid in the intensification of plant immune responses and disease control.
Key words: Bacillus amyloliquefaciens, disease control, plant immune response, sodium silicate