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POSTERS: Proteomics/Metabolomics/Genomics I

P-579 - Elucidating gene expression patterns during hormetic stimulation in Globisporangium.

Monday, August 4, 2025
4:30 PM - 5:15 PM HST
Location: Exhibit Hall 3

    Author(s)

  • MR

    Michael Rosengrant

    Delaware Valley University
    Doylestown, Pennsylvania, United States

  • DB

    Danielle Bowman

    Delaware Valley University
    Doylestown, Pennsylvania, United States

  • AA

    Ameer Awawda

    Undergraduate Research Intern
    Delaware Valley University
    Doylestown, Pennsylvania, United States

  • IM

    Isabel Molineros

    Delaware Valley University
    Doylestown, PA, USA

    • Presenting Author(s)

  • CG

    Carla D. Garzon (she/her/hers)

    K.H. Littlefield Endowed Professor of Plant Science
    Delaware Valley University
    Doylestown, Pennsylvania, United States

Abstract Text: Chemical hormesis has been observed in oomycetes in response to subinhibitory doses of fungicides. Previous studies demonstrated that the radial growth of some Pythium spp., Globisporangium spp., and Phytophthora spp. was significantly stimulated in vitro or in planta. Although significant progress has been made regarding the identification of pathosystems that present hormetic stimulation, the metabolic pathways involved in these responses have not been fully elucidated. The goals of this study were to identify Globisporangium isolates that demonstrated significant stimulatory responses to subinhibitory doses of mefenoxam, and to examine the differential gene expression during stimulatory responses. Isolates were grown on water agar amended with 10 subinhibitory doses of mefenoxam for 96 hrs, with radial growth assessments every 24 hrs, to determine the maximum stimulatory dose (MSD). The isolate that exhibited the most stimulation was selected for differential gene expression analysis. Mycelial plugs were transferred to one quarter strength PDB amended with the MSD dose and a fungicide free control. Total RNA was purified and cDNA was synthesized. Differential gene expression analysis was conducted, followed by RT-qPCR validation. We report our findings and discuss potential mechanisms of hormetic stimulation.